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Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina
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Neurociência
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JoVE Journal Neurociência
Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina

4,204 Views

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09:50 min

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December 25, 2021

DOI:

10.3791/63038-v

DOI:
10.3791/63038-v

•

09:50 min
December 25, 2021

•

14 Views
, , , ,
1Molecular Biology, Genetics and Bioengineering Program,Sabanci University, 2Nanotechnology Research and Application Center (SUNUM),Sabanci University

Summary

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This protocol presents how to quantify AAV transduction efficiency in mouse retina using digital droplet PCR (dd-PCR) together with small scale AAV production, intravitreal injection, retinal imaging, and retinal genomic DNA isolation.

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Digital Droplet PCRAAV TransductionMurine RetinaQuantificationAbsolute QuantificationCRISPRGene TherapyMitochondrial DNATransfectionVirus Purification

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Cite this Article

Okan, I. C. T., Ahmadian, M., Tutuncu, Y., Altay, H. Y., Agca, C. Digital Droplet PCR Method for the Quantification of AAV Transduction Efficiency in Murine Retina. J. Vis. Exp. (178), e63038, doi:10.3791/63038 (2021).

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