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Surveillance en temps réel de la respiration mitochondriale dans les cellules T primaires humaines différenciées par cytokines
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells
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Surveillance en temps réel de la respiration mitochondriale dans les cellules T primaires humaines différenciées par cytokines

3,536 Views

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06:55 min

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October 19, 2021

DOI:

10.3791/62984-v

DOI:
10.3791/62984-v

•

06:55 min
October 19, 2021

•

3 Views
, , , , , ,
1National Center for Cancer Immune Therapy, Department of Oncology,University Hospital Herlev, 2Department of Cellular and Molecular Medicine, Center for Healthy Aging,University of Copenhagen, 3Department of Immunology and Microbiology, Inflammation and Cancer Group,University of Copenhagen

Summary

Automatically generated

L’adaptation métabolique est fondamentale pour les lymphocytes T car elle dicte la différenciation, la persistance et la cytotoxicité. Ici, un protocole optimisé pour surveiller la respiration mitochondriale dans les cellules T primaires humaines différenciées par cytokines ex vivo est présenté.

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Cite this Article

Mølgaard, K., Rahbech, A., Met, Ö., Svane, I. M., thor Straten, P., Desler, C., Peeters, M. J. W. Real-time Monitoring of Mitochondrial Respiration in Cytokine-differentiated Human Primary T Cells. J. Vis. Exp. (176), e62984, doi:10.3791/62984 (2021).

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