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Peptidomik Çalışmalar için Aminlerin Indirgeyici Metilasyonunu Kullanarak Numune Hazırlama ve Göreli Nicellik

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

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Peptidomik Çalışmalar için Aminlerin Indirgeyici Metilasyonunu Kullanarak Numune Hazırlama ve Göreli Nicellik

2,069 Views

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08:00 min

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November 04, 2021

DOI:

10.3791/62971-v

DOI:

10.3791/62971-v

•

08:00 min

November 04, 2021

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1 Views

Claudia Neves Correa², Louise Oliveira Fiametti, Maria Eduarda Mazzi Esquinca, Leandro Mantovani de Castro²

¹Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), ²Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Tags

Sample Preparation Relative Quantitation Reductive Methylation Amines Peptidomics Protease Inactivation Isotopic Labeling Neuroblastoma Cells Zebrafish Brain Cellular Lysate Ultrafiltration Solid-phase Extraction

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