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ペプチド学研究におけるアミンの還元メチル化を用いたサンプル調製と相対定量

JoVE Journal
Bioquímica

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JoVE Journal Bioquímica

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

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ペプチド学研究におけるアミンの還元メチル化を用いたサンプル調製と相対定量

2,084 Views

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08:00 min

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November 04, 2021

DOI:

10.3791/62971-v

DOI:

10.3791/62971-v

•

08:00 min

November 04, 2021

•

10 Views

Claudia Neves Correa^1,2, Louise Oliveira Fiametti¹, Maria Eduarda Mazzi Esquinca¹, Leandro Mantovani de Castro^1,2

¹Department of Biological and Environmental Sciences, Bioscience Institute,Sao Paulo State University (UNESP), ²Biodiversity of Coastal Environments Postgraduate Program, Bioscience Institute,Sao Paulo State University (UNESP)

Summary

この記事では、熱不活性化に基づくサンプル調製方法を、後分析による分解を回避する内因性ペプチドを保存し、続いて同位体標識とLC-MSを使用した相対定量を行う方法について説明します。

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