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在生物安全3级（BSL-3）条件下使胃肠道类器官适应病原体感染和单细胞测序
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September 10, 2021
DOI:
1Department of Infectious Diseases, Molecular Virology,Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine,University of Florida, Gainesville, 3Department of Infectious Diseases, Virology,Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”,German Cancer Research Center (DKFZ)
Summary
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该协议描述了如何从根尖或基底外侧感染人肠道类器官，以使用单细胞RNA测序（scRNAseq）技术在单细胞水平上表征宿主/病原体相互作用。
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Stanifer, M. L., Boulant, S. Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions. J. Vis. Exp. (175), e62857, doi:10.3791/62857 (2021).
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