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Aislamiento de neutrófilos humanos de sangre entera y buffy coats
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation of Human Neutrophils from Whole Blood and Buffy Coats
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Aislamiento de neutrófilos humanos de sangre entera y buffy coats

15,388 Views

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08:12 min

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September 17, 2021

DOI:

10.3791/62837-v

DOI:
10.3791/62837-v

•

08:12 min
September 17, 2021

•

68 Views
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1Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science,Mahidol University

Summary

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Este protocolo detalla un método para el aislamiento de neutrófilos de sangre total, capas buffy o membranas de leucoféresis, logrando un buen rendimiento, alta pureza y mínima activación celular. Utilizamos la purificación por gradiente, la sedimentación de glóbulos rojos (RBC) y la lisis de RBC para obtener una preparación de neutrófilos de alta calidad / pureza.

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Cite this Article

Hsu, A. Y., Peng, Z., Luo, H., Loison, F. Isolation of Human Neutrophils from Whole Blood and Buffy Coats. J. Vis. Exp. (175), e62837, doi:10.3791/62837 (2021).

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