This content is Free Access.
Chapters
- 00:04Introduction
- 00:45Gradient Layering
- 02:40Erythrocyte Sedimentation
- 03:45Residual RBC Lysis
- 05:06Quality Determination of Isolated Neutrophil
- 05:44Results: Neutrophil Yield and Quality Estimation
- 07:34Conclusion
This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.
