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Isolation of Human Neutrophils from Whole Blood and Buffy Coats
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Biologia
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JoVE Journal Biologia
Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

15,648 Views

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08:12 min

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September 17, 2021

DOI:

10.3791/62837-v

DOI:
10.3791/62837-v

•

08:12 min
September 17, 2021

•

68 Views
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1Department of Pathology,Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, 2Department of Microbiology, Faculty of Science,Mahidol University

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This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.

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Cite this Article

Hsu, A. Y., Peng, Z., Luo, H., Loison, F. Isolation of Human Neutrophils from Whole Blood and Buffy Coats. J. Vis. Exp. (175), e62837, doi:10.3791/62837 (2021).

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