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肌萎缩性侧索硬化症模型中突触功能的实时荧光测量

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

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肌萎缩性侧索硬化症模型中突触功能的实时荧光测量

DOI:

10.3791/62813-v

•

08:59 min

•

July 16, 2021

•

Karthik Krishnamurthy*¹, Davide Trotti, Piera Pasinelli, Brigid Jensen*¹

¹Jefferson Weinberg ALS Center,Thomas Jefferson University

Capítulos

00:04Introduction
00:52Microscope and Perfusion System Setup
02:33Styryl Dye Imaging of Synaptic Vesicle Release
04:30Fluorescence Imaging of Gcamp6m Calcium Transients
05:36Image Analysis
06:23Results: Real-Time Monitoring of the Dynamics of Presynaptic Calcium Influx and Synaptic Vesicle Membrane Fusion in In Vitro Cultured Neurons
08:15Conclusion

Summary

Tadução automática

描述了两种相关方法，以可视化突触传递所需的亚细胞事件。这些方案能够使用体外培养神经元的活细胞成像实时监测突触前钙流入和突触囊泡膜融合的动态。

Tags

Keywords: Real-time Fluorescent Synaptic Amyotrophic Lateral Sclerosis Synaptic Function Electrophysiology Primary Rodent Neuronal Cultures Induced Pluripotent Stem Cells GCaMP Styryl Dye ACSF Potassium Chloride Confocal Imaging

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