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دليل لطخة والغطس تجميد العينات البيولوجية للجسيمات المفردة المجهر الإلكترون المبردة

JoVE Journal
Bioquímica
Author Produced

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JoVE Journal Bioquímica

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

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دليل لطخة والغطس تجميد العينات البيولوجية للجسيمات المفردة المجهر الإلكترون المبردة

5,398 Views

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09:16 min

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February 07, 2022

DOI:

10.3791/62765-v

DOI:

10.3791/62765-v

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09:16 min

February 07, 2022

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6 Views

Hoang P. M. Nguyen*¹, Kelly L. McGuire*¹, Brian D. Cook*¹, Mark A. Herzik, Jr.

¹Department of Chemistry and Biochemistry,University of California, San Diego

Tags

Manual Blot-and-plunge Freezing Biological Specimens Single-particle Cryogenic Electron Microscopy Transmission Electron Microscopy Cryogen Dewar Manual Plunger Ethane Vessel Plunging Arm Cryogen Preparation Liquid Nitrogen Ethane Gas

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