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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Biochimie
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Journal JoVE Biochimie
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

DOI:
10.3791/62765-v

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09:16 min

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February 07, 2022

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, , ,
1Department of Chemistry and Biochemistry,University of California, San Diego

Chapitres

  • 00:04Introduction
  • 00:22Prepare the Manual Plunging Environment
  • 00:42Prepare the Cryogen Dewar and Manual Plunger
  • 02:04Prepare the Cryogen
  • 04:36Prepare Plunging Materials and Accessories
  • 05:13Prepare CryoEM Specimens by Plunge Freezing
  • 07:22Representative Results
  • 07:50Conclusion

Summary

Traduction automatique

Traduction automatique

This manuscript outlines the blot-and-plunge method to manually freeze biological specimens for single-particle cryogenic electron microscopy.

Tags

Manual Blot-and-plunge FreezingBiological SpecimensSingle-particle Cryogenic Electron MicroscopyTransmission Electron MicroscopyCryogen DewarManual PlungerEthane VesselPlunging ArmCryogen PreparationLiquid NitrogenEthane Gas

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