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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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February 07, 2022
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This manuscript outlines the blot-and-plunge method to manually freeze biological specimens for single-particle cryogenic electron microscopy.
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Nguyen, H. P. M., McGuire, K. L., Cook, B. D., Herzik, Jr., M. A. Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy. J. Vis. Exp. (180), e62765, doi:10.3791/62765 (2022).
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