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Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy
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Bioquímica
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JoVE Journal Bioquímica
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

5,398 Views

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09:16 min

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February 07, 2022

DOI:

10.3791/62765-v

DOI:
10.3791/62765-v

•

09:16 min
February 07, 2022

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6 Views
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1Department of Chemistry and Biochemistry,University of California, San Diego

Tags

Manual Blot-and-plunge FreezingBiological SpecimensSingle-particle Cryogenic Electron MicroscopyTransmission Electron MicroscopyCryogen DewarManual PlungerEthane VesselPlunging ArmCryogen PreparationLiquid NitrogenEthane Gas

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Nguyen, H. P. M., McGuire, K. L., Cook, B. D., Herzik, Jr., M. A. Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy. J. Vis. Exp. (180), e62765, doi:10.3791/62765 (2022).

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