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使用脑和脊柱联合手术用双病毒载体靶向新生大鼠的皮质脊髓束

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery

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使用脑和脊柱联合手术用双病毒载体靶向新生大鼠的皮质脊髓束

1,841 Views

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07:27 min

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June 30, 2021

DOI:

10.3791/62698-v

DOI:

10.3791/62698-v

•

07:27 min

June 30, 2021

•

10 Views

Rupert D. Smit¹, Thomas J. Campion III¹, Rachel L. Stingel¹, Pushti H. Shah¹, Jie Chen¹, George M. Smith¹

¹Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine,Temple University

Summary

该协议展示了一种通过将顺行性化学遗传修饰剂注射到体细胞运动皮层和逆行可运输的Cre重组酶到颈脊髓中，将基因疗法应用于出生后5-10天新生大鼠细胞亚群的新方法。

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