需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery
1,836 Views
•
07:27 min
•
June 30, 2021
DOI:
Summary
Automatically generated
This protocol demonstrates a novel method for applying gene therapies to subpopulations of cells in neonatal rats at postnatal ages 5-10 days by injecting an anterograde chemogenetic modifier into the somatomotor cortex and a retrogradely transportable Cre recombinase into the cervical spinal cord.
Read Article
Cite this Article
Smit, R. D., Campion III, T. J., Stingel, R. L., Shah, P. H., Chen, J., Smith, G. M. Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery. J. Vis. Exp. (172), e62698, doi:10.3791/62698 (2021).
Copy