/
/
Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery
Capítulos
- 00:04Introduction
- 01:00Performing the Craniotomy and Exposing the Somatomotor Cortex
- 02:21Injecting Virus into the Somatomotor Cortex
- 03:00Creating a Spinal Window for Precise Spinal Cord Injections
- 05:31Direct Injections into the Spinal Cord Targeting Axon Terminals
- 06:33Results: Transduction of Neurons in the Motor Cortex
- 06:52Conclusion
This protocol demonstrates a novel method for applying gene therapies to subpopulations of cells in neonatal rats at postnatal ages 5-10 days by injecting an anterograde chemogenetic modifier into the somatomotor cortex and a retrogradely transportable Cre recombinase into the cervical spinal cord.
