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Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
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Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
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07:15 min
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November 22, 2021
DOI:
1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology,Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital,Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences,University of Chinese Academy of Sciences
Summary
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Here we describe a protocol for pseudovirus packaging and the measurement of antibody neutralizing activity.
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Chang, X., Zhou, K., Liu, Y., Duan, L., Zhang, L., Zhang, G., Wang, H., Wang, G. Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity. J. Vis. Exp. (177), e62626, doi:10.3791/62626 (2021).
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