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Immunology and Infection

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JoVE Journal Immunology and Infection
Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity
 

Preparation of Pseudo-Typed H5 Avian Influenza Viruses with Calcium Phosphate Transfection Method and Measurement of Antibody Neutralizing Activity

Article DOI: 10.3791/62626-v • 07:15 min • November 22nd, 2021
November 22nd, 2021 •
*1, *1, 1,2, 3, 2, 3, 4, 1
1Nanjing Advanced Academy of Life and Health, 2Institute of Animal Bio-technology, Jilin Academy of Agricultural Sciences, 3National Clinical Research Center for Infectious Diseases, Shenzhen Third People’s Hospital, Southern University of Science and Technology, 4CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, University of Chinese Academy of Sciences
* These authors contributed equally

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Here we describe a protocol for pseudovirus packaging and the measurement of antibody neutralizing activity.

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Pseudo-Typed H5 Avian Influenza Viruses Calcium Phosphate Transfection Method Antibody Neutralizing Activity Pseudo Virus Preparations Pseudo Virus Neutralization Assays Troubleshooting Modifications HEK293 FT Cells Complete DMEM Medium Chloroquine Reagents Plasmid DNA Supernatant Hemagglutinin Protein Expression PBS 96 Well Round Bottom Plate
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