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一种人外周血淋巴细胞γ-H2AX病灶测定的自动显微镜评分方法

JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer

An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes

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一种人外周血淋巴细胞γ-H2AX病灶测定的自动显微镜评分方法

4,305 Views

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08:23 min

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December 25, 2021

DOI:

10.3791/62623-v

DOI:

10.3791/62623-v

•

08:23 min

December 25, 2021

•

5 Views

Shankari Nair¹, Samantha Cairncross², Xanthene Miles¹, Monique Engelbrecht³, Peter du Plessis¹, Julie Bolcaen¹, Randall Fisher¹, Roya Ndimba¹, Charnay Cunningham⁴, Wilner Martínez-López⁵, Christian Schunck⁶, Charlot Vandevoorde¹

¹Radiation Biophysics Division, Nuclear Medicine Department,iThemba LABS, ²Department of Biotechnology,University of the Western Cape, ³Department of Medical Biosciences,University of the Western Cape, ⁴Division of Medical Physiology, Department of Medicine and Health Sciences,Stellenbosch University, ⁵Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine,University of the Republic, ⁶MetaSystems Hard & Software GmbH

Summary

该协议介绍了外周血淋巴细胞上γ-H2AX病灶测定的载玻片制备和自动评分。为了说明测定的方法和灵敏度，在体外照射分离的淋巴细胞。这种自动化的DNA DSB检测方法对于快速和高通量的生物剂量测定应用非常有用。

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