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유전자 변형 된 Anopheline 모기 인구에서 인델 돌연변이를 감지하는 디지털 물방울 PCR
JoVE Journal
Genética
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JoVE Journal Genética
Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations
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유전자 변형 된 Anopheline 모기 인구에서 인델 돌연변이를 감지하는 디지털 물방울 PCR

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05:51 min

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June 28, 2021

DOI:

10.3791/62607-v

DOI:
10.3791/62607-v

•

05:51 min
June 28, 2021

•

3 Views
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1Department of Microbiology & Molecular Genetics,University of California, Irvine, 2Department of Molecular Biology & Biochemistry,University of California, Irvine

Tags

Digital Droplet PCRDdPCRNHEJ AnalysisIndel MutationsGene-edited MosquitoGenetic VariationHigh ThroughputFast TurnaroundField AnalysisGenetically Modified Organisms

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Cite this Article

Carballar-Lejarazú, R., Pham, T. B., Kelsey, A., Tushar, T., James, A. A. Digital-Droplet PCR to Detect Indels Mutations in Genetically Modified Anopheline Mosquito Populations. J. Vis. Exp. (172), e62607, doi:10.3791/62607 (2021).

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