ジャーナル
/
遺伝学
/
Microinjection Method for Anopheles gambiae Embryos
JoVE Journal
遺伝学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 遺伝学
Microinjection Method for Anopheles gambiae Embryos

Microinjection Method for Anopheles gambiae Embryos

4,156 Views

•

05:39 min

•

July 07, 2021

DOI:

10.3791/62591-v

DOI:
10.3791/62591-v

•

05:39 min
July 07, 2021

•

15 Views
, , ,
1Department of Microbiology & Molecular Genetics,University of California, Irvine, 2Department of Molecular Biology & Biochemistry,University of California, Irvine

概要

Automatically generated

Microinjection techniques are essential to introduce exogenous genes into the genomes of mosquitoes. This protocol explains a method used by the James laboratory to microinject DNA constructs into Anopheles gambiae embryos to generate transformed mosquitoes.

Explore More Videos

MicroinjectionAnopheles GambiaeEmbryosTransgenicMalariaPopulation SuppressionMosquito SpeciesAspiratorConical TubeDental FilmNylon MeshFilter PaperIncubatorForcepsPetri DishMembraneFilter PaperMicroloader TipNeedle HolderAutomated Pressure Pump

Read Article

Cite this Article

Carballar-Lejarazú, R., Tushar, T., Pham, T. B., James, A. A. Microinjection Method for Anopheles gambiae Embryos. J. Vis. Exp. (173), e62591, doi:10.3791/62591 (2021).

Download .ris file

Copy

Share Video

.

Copy