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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
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Bioquímica
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JoVE Journal Bioquímica
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

3,765 Views

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09:19 min

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June 01, 2022

DOI:

10.3791/62547-v

DOI:
10.3791/62547-v

•

09:19 min
June 01, 2022

•

4 Views
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1Department of Molecular Biology and Biochemistry,University of California Irvine, 2Department of Ecology and Evolutionary Biology,University of California Irvine, 3Entech Instruments Inc.

Summary

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This protocol describes the extraction of volatile organic compounds from a biological sample with the vacuum-assisted sorbent extraction method, gas chromatography coupled with mass spectrometry using the Entech Sample Preparation Rail, and data analysis. It also describes culture of biological samples and stable isotope probing.

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Phan, J., Kapcia III, J., Rodriguez, C. I., Vogel, V. L., Cardin, D. B., Dunham, S. J. B., Whiteson, K. Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction. J. Vis. Exp. (184), e62547, doi:10.3791/62547 (2022).

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