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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

3,765 Views

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09:19 min

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June 01, 2022

DOI:

10.3791/62547-v

DOI:
10.3791/62547-v

•

09:19 min
June 01, 2022

•

4 Views
, , , , , ,
1Department of Molecular Biology and Biochemistry,University of California Irvine, 2Department of Ecology and Evolutionary Biology,University of California Irvine, 3Entech Instruments Inc.

概要

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This protocol describes the extraction of volatile organic compounds from a biological sample with the vacuum-assisted sorbent extraction method, gas chromatography coupled with mass spectrometry using the Entech Sample Preparation Rail, and data analysis. It also describes culture of biological samples and stable isotope probing.

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Cite this Article

Phan, J., Kapcia III, J., Rodriguez, C. I., Vogel, V. L., Cardin, D. B., Dunham, S. J. B., Whiteson, K. Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction. J. Vis. Exp. (184), e62547, doi:10.3791/62547 (2022).

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