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標的ナノ粒子の研究のための乳癌の同質性マウスモデルからの原発癌関連線維芽細胞の分離
JoVE Journal
Pesquisa do Câncer
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JoVE Journal Pesquisa do Câncer
Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles
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標的ナノ粒子の研究のための乳癌の同質性マウスモデルからの原発癌関連線維芽細胞の分離

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08:02 min

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May 14, 2021

DOI:

10.3791/62504-v

DOI:
10.3791/62504-v

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08:02 min
May 14, 2021

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1Istituti Clinici Scientifici Maugeri IRCCS, 2Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”,Università di Milano, 3Dipartimento di Biotecnologie e Bioscienze,Università di Milano-Bicocca

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Primary Cancer-associated FibroblastsMurine Breast CancerTumor MicroenvironmentIn Vitro StudiesTumor StromaTumor DissociationCell IsolationDead Cell RemovalMagnetic SeparationCell Culture

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Truffi, M., Sitia, L., Sevieri, M., Bonizzi, A., Rizzuto, M. A., Mazzucchelli, S., Corsi, F. Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles. J. Vis. Exp. (171), e62504, doi:10.3791/62504 (2021).

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