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使用夹层冷冻装置快速冷冻,在电子显微镜下对生物标本进行良好的超微结构保存
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
DOI:

09:03 min

July 19, 2021

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Capítulos

  • 00:04Introduction
  • 00:48Rapid Freezing of Cell Suspensions for Freeze-Substitution
  • 07:19Results: Ultrastructural Preservation of Biological Specimens in Electron Microscopy Using Sandwich Freezing Device
  • 08:03Conclusion

Summary

Tadução automática

在这里,我们将展示如何使用三明治冷冻装置快速冷冻生物标本,包括细菌,酵母,培养细胞,分离细胞,动物和人体组织以及病毒。我们还展示了如何在快速冷冻后为超薄切片制备试样。

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