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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
Capítulos
- 00:04Introduction
- 00:48Rapid Freezing of Cell Suspensions for Freeze-Substitution
- 07:19Results: Ultrastructural Preservation of Biological Specimens in Electron Microscopy Using Sandwich Freezing Device
- 08:03Conclusion
Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.
