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Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy
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Biologia
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JoVE Journal Biologia
Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

3,813 Views

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09:03 min

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July 19, 2021

DOI:

10.3791/62431-v

DOI:
10.3791/62431-v

•

09:03 min
July 19, 2021

•

23 Views
, , , , ,
1Medical Mycology Research Center,Chiba University, 2Marine Works Japan Ltd.

Summary

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Here we show how to use the sandwich freezing device for rapid freezing of biological specimens, including bacteria, yeast, cultured cells, isolated cells, animal and human tissues, and viruses. We also show how to prepare specimens for ultrathin sectioning after rapid freezing.

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Cite this Article

Yamaguchi, M., Takahashi-Nakaguchi, A., Uematsu, K., Taguchi, M., Sato-Okamoto, M., Chibana, H. Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy. J. Vis. Exp. (173), e62431, doi:10.3791/62431 (2021).

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