Production of Human CRISPR-Engineered CAR-T Cells

Sangya Agarwal; Nils Wellhausen; Bruce L. Levine; Carl H. June

doi:10.3791/62299

Journal

/

Imunologia e Infecção

/

Herstellung von humanen CRISPR-engineered CAR-T Zellen

JoVE Journal
Imunologia e Infecção

É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.

JoVE Journal Imunologia e Infecção

Production of Human CRISPR-Engineered CAR-T Cells

Please note that all translations are automatically generated. Click here for the English version.

Herstellung von humanen CRISPR-engineered CAR-T Zellen

11,711 Views

•

06:33 min

•

March 15, 2021

DOI:

10.3791/62299-v

DOI:

10.3791/62299-v

•

06:33 min

March 15, 2021

•

33 Views

Sangya Agarwal*^1,2, Nils Wellhausen*^1,2, Bruce L. Levine^1,2, Carl H. June^1,2

¹Center for Cellular Immunotherapies, Perelman School of Medicine,University of Pennsylvania, ²Parker Institute for Cancer Immunotherapy,University of Pennsylvania

Summary

Hier stellen wir ein Protokoll für die Genbearbeitung in primären menschlichen T-Zellen vor, das die CRISPR Cas-Technologie verwendet, um CAR-T-Zellen zu modifizieren.

Explore More Videos

CRISPR-Cas9 CAR T Cell Therapy Genome Editing Human Clinical Trials PBMC CD4 T Cells CD8 T Cells IL-7 IL-15 Ribonucleoprotein (RNP) Complex Electroporation T-cell Activation And Expansion Sequencing Primers

Read Article

Herstellung von humanen CRISPR-engineered CAR-T Zellen

Summary

Automatically generated

Explore More Videos