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Production of Human CRISPR-Engineered CAR-T Cells
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JoVE Journal 免疫学と感染
Production of Human CRISPR-Engineered CAR-T Cells

Production of Human CRISPR-Engineered CAR-T Cells

DOI:
10.3791/62299-v

•

06:33 min

•

March 15, 2021

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, , ,
1Center for Cellular Immunotherapies, Perelman School of Medicine,University of Pennsylvania, 2Parker Institute for Cancer Immunotherapy,University of Pennsylvania

章

  • 00:05Introduction
  • 01:03Designing of sgRNAs and Gene Disruption in Primary Human T-Cells
  • 03:23CRISPR Efficiency: Sequencing and Indel Detection
  • 04:13Results: Manufacturing Human CAR-T Cells Using CRISPR Cas9 Edited T-Cells
  • 05:30Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol for gene editing in primary human T cells using CRISPR Cas Technology to modify CAR-T cells.

タグ

CRISPR-Cas9CAR T Cell TherapyGenome EditingHuman Clinical TrialsPBMCCD4 T CellsCD8 T CellsIL-7IL-15Ribonucleoprotein (RNP) ComplexElectroporationT-cell Activation And ExpansionSequencing Primers

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