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Production of Human CRISPR-Engineered CAR-T Cells
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JoVE Journal Immunologia e infezioni
Production of Human CRISPR-Engineered CAR-T Cells

Production of Human CRISPR-Engineered CAR-T Cells

11,769 Views

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06:33 min

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March 15, 2021

DOI:

10.3791/62299-v

DOI:
10.3791/62299-v

•

06:33 min
March 15, 2021

•

33 Views
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1Center for Cellular Immunotherapies, Perelman School of Medicine,University of Pennsylvania, 2Parker Institute for Cancer Immunotherapy,University of Pennsylvania

Summary

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Here, we present a protocol for gene editing in primary human T cells using CRISPR Cas Technology to modify CAR-T cells.

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Cite this Article

Agarwal, S., Wellhausen, N., Levine, B. L., June, C. H. Production of Human CRISPR-Engineered CAR-T Cells. J. Vis. Exp. (169), e62299, doi:10.3791/62299 (2021).

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