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인간 CRISPR-엔지니어링 CAR-T 셀 생산
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Production of Human CRISPR-Engineered CAR-T Cells
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인간 CRISPR-엔지니어링 CAR-T 셀 생산

11,701 Views

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06:33 min

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March 15, 2021

DOI:

10.3791/62299-v

DOI:
10.3791/62299-v

•

06:33 min
March 15, 2021

•

33 Views
, , ,
1Center for Cellular Immunotherapies, Perelman School of Medicine,University of Pennsylvania, 2Parker Institute for Cancer Immunotherapy,University of Pennsylvania

Summary

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여기서, 우리는 CAR-T 세포를 수정하기 위하여 CRISPR Cas 기술을 사용하여 1 차적인 인간 T 세포에 있는 유전자 편집을 위한 프로토콜을 제시합니다.

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Cite this Article

Agarwal, S., Wellhausen, N., Levine, B. L., June, C. H. Production of Human CRISPR-Engineered CAR-T Cells. J. Vis. Exp. (169), e62299, doi:10.3791/62299 (2021).

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