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A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
 

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

Article DOI: 10.3791/62268-v • 06:54 min • June 3rd, 2021
June 3rd, 2021 •
1, 1
1Department of Anatomy, School of Biomedical Sciences, University of Otago

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We report a simple, time-efficient and high-throughput fluorescence spectroscopy-based assay for the quantification of actin filaments in ex vivo biological samples from brain tissues of rodents and human subjects.

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Fluorescence Spectroscopy-based Assay Actin Polymerization Rodent Brain Tissues Human Brain Tissues Cytoskeleton Cellular Morphology Cellular Physiology Neurons Actin-binding Proteins Post-translational Regulation Interneuronal Synaptic Contacts F-actin Levels Signaling And Plasticity Neuropathological States Fluorescent Analogs Of Phalloidin Filamentous Actin Pathophysiological States Fluorometric Assay Ex-vivo Biological Samples Wholesale Homogenates Biochemically Isolated Synaptic Terminals
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