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A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay
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Neurociência
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JoVE Journal Neurociência
A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay

A Caenorhabditis elegans Nutritional-status Based Copper Aversion Assay

DOI:
10.3791/55939-v

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06:45 min

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July 26, 2017

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, ,
1Department of Biology,Queen’s University, 2Centre for Neuroscience,Queen’s University

Capítulos

  • 00:05Título
  • 00:42Preparation of Experimental Organisms
  • 01:15Preparation of Assay Plates
  • 02:31Chemotaxis Assay
  • 05:31Results: npr-9 Overexpression Nematodes Do Not Initiate Aversion to Copper
  • 06:21Conclusion

Summary

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Here, we present a Caenorhabditis elegans-specific assay designed to evaluate changes in copper aversion behavior and the ability to locate a common food source, as the organism progresses from a well-fed to starved nutritional state.

Tags

Caenorhabditis ElegansCopper Aversion AssayNutritional StatusBehavioral NeuroscienceL4 Stage NematodesOP50 E. ColiCopper(II)sulfateFood PatchMidline Barrier

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