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Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
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Biologia
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JoVE Journal Biologia
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

4,951 Views

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11:03 min

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March 09, 2021

DOI:

10.3791/62248-v

DOI:
10.3791/62248-v

•

11:03 min
March 09, 2021

•

17 Views
, ,
1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences,University of California, 2Chan Zuckerberg Biohub

Summary

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Water-in-oil droplet assays are useful for analytical chemistry, enzyme evolution, and single cell analysis, but typically require microfluidics to form the droplets. Here, we describe particle templated emulsification, a microfluidic-free approach to perform droplet assays.

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Weisgerber, D. W., Hatori, M. N., Abate, A. R. Particle Templated Emulsification enables Microfluidic-Free Droplet Assays. J. Vis. Exp. (169), e62248, doi:10.3791/62248 (2021).

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