Water-in-oil droplet assays are useful for analytical chemistry, enzyme evolution, and single cell analysis, but typically require microfluidics to form the droplets. Here, we describe particle templated emulsification, a microfluidic-free approach to perform droplet assays.
Weisgerber, D. W., Hatori, M. N., Abate, A. R. Particle Templated Emulsification enables Microfluidic-Free Droplet Assays. J. Vis. Exp. (169), e62248, doi:10.3791/62248 (2021).