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Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method
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Neurociência
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JoVE Journal Neurociência
Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

DOI:
10.3791/62178-v

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09:01 min

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March 06, 2021

•

,
1Eye Research Institute,Oakland University

Capítulos

  • 00:04Introduction
  • 00:36Tissue Preparation
  • 03:04Immunostaining and Refractive Index Matching
  • 04:05Mounting
  • 05:30Imaging
  • 06:36Results: Improved 3D Resolution of Fine Processes of Retina by Modified CLARITY Tissue Clearing Method
  • 08:39Conclusion

Summary

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Here we present a protocol to adapt the CLARITY method of the brain tissues for whole-mount retinas to improve the quality of standard immunohistochemical staining and high-resolution imaging of retinal neurons and their subcellular structures.

Tags

ImmunostainingWhole-mount RetinaRetinal NeuronsMorphologyOptical Transparency3D ImagingCircuit WiringSubcellular StructuresEnucleationCorneaLensScleraRetina IsolationNitrocellulose FilterA4P0 SolutionSDSTriton X-100Primary AntibodyImmunostaining

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