需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method
6,892 Views
•
09:01 min
•
March 06, 2021
DOI:
Summary
Automatically generated
Here we present a protocol to adapt the CLARITY method of the brain tissues for whole-mount retinas to improve the quality of standard immunohistochemical staining and high-resolution imaging of retinal neurons and their subcellular structures.
Read Article
Cite this Article
Alessio, E. J., Zhang, D. Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method. J. Vis. Exp. (169), e62178, doi:10.3791/62178 (2021).
Copy