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Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

6,892 Views

09:01 min

March 06, 2021

DOI:

09:01 min
March 06, 2021

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Summary

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Here we present a protocol to adapt the CLARITY method of the brain tissues for whole-mount retinas to improve the quality of standard immunohistochemical staining and high-resolution imaging of retinal neurons and their subcellular structures.

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