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클릭 화학을 이용한 지방 아실화 단백질의 검출을 위한 알키닐 지방산 유사체의 최적화된 혼입
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
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클릭 화학을 이용한 지방 아실화 단백질의 검출을 위한 알키닐 지방산 유사체의 최적화된 혼입

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07:27 min

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April 09, 2021

DOI:

10.3791/62107-v

DOI:
10.3791/62107-v

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07:27 min
April 09, 2021

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9 Views
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1Department of Biology,University of Waterloo

Tags

Alkynyl Fatty Acid AnalogsClick ChemistryFatty Acylated ProteinsFatty Acid LabelingMetabolic LabelingHEK-293 T-cellsBSA-bound Fatty AcidsSaponificationPotassium HydroxideFatty Acid-free BSA

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Cite this Article

Liao, L. M. Q., Gray, R. A. V., Martin, D. D. O. Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry. J. Vis. Exp. (170), e62107, doi:10.3791/62107 (2021).

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