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Geoptimaliseerde integratie van alkynylvetzuuranalogen voor de detectie van vetacyleiwitten met behulp van Click Chemistry
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry
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Geoptimaliseerde integratie van alkynylvetzuuranalogen voor de detectie van vetacyleiwitten met behulp van Click Chemistry

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07:27 min

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April 09, 2021

DOI:

10.3791/62107-v

DOI:
10.3791/62107-v

•

07:27 min
April 09, 2021

•

9 Views
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1Department of Biology,University of Waterloo

Tags

Alkynyl Fatty Acid AnalogsClick ChemistryFatty Acylated ProteinsFatty Acid LabelingMetabolic LabelingHEK-293 T-cellsBSA-bound Fatty AcidsSaponificationPotassium HydroxideFatty Acid-free BSA

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Liao, L. M. Q., Gray, R. A. V., Martin, D. D. O. Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry. J. Vis. Exp. (170), e62107, doi:10.3791/62107 (2021).

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