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Optimeret inkorporering af alkynylfedtsyreanaloger til påvisning af fedtaylerede proteiner ved hjælp af klikkemi
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry

Optimeret inkorporering af alkynylfedtsyreanaloger til påvisning af fedtaylerede proteiner ved hjælp af klikkemi

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07:27 min

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April 09, 2021

DOI:

10.3791/62107-v

DOI:
10.3791/62107-v

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07:27 min
April 09, 2021

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9 Views
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1Department of Biology,University of Waterloo

Tags

Alkynyl Fatty Acid AnalogsClick ChemistryFatty Acylated ProteinsFatty Acid LabelingMetabolic LabelingHEK-293 T-cellsBSA-bound Fatty AcidsSaponificationPotassium HydroxideFatty Acid-free BSA

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Liao, L. M. Q., Gray, R. A. V., Martin, D. D. O. Optimized Incorporation of Alkynyl Fatty Acid Analogs for the Detection of Fatty Acylated Proteins using Click Chemistry. J. Vis. Exp. (170), e62107, doi:10.3791/62107 (2021).

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