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使用多体分析在发育中的鼠标大脑中进行翻译分析
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Neurociência
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JoVE Journal Neurociência
Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
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使用多体分析在发育中的鼠标大脑中进行翻译分析

DOI:
10.3791/62088-v

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08:38 min

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May 22, 2021

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, ,
1Department of Biochemistry and Molecular Biology; Cumming School of Medicine,University of Calgary, 2Department of Medical Genetics, Cumming School of Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary

Capítulos

  • 00:04Introduction
  • 00:50Preparation of Sucrose Gradient
  • 01:57Tissue Dissection
  • 03:05Cell Lysis
  • 04:34Fractionation and Sample Collection
  • 06:15Results: Polysome Profiling Analysis of the Developing Mouse Cortex
  • 07:39Conclusion

Summary

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Tadução automática

哺乳动物大脑的发展需要在翻译水平上正确控制基因表达。在这里，我们描述了一个多体分析系统，该系统具有易于组装的蔗糖梯度制作和分馏平台，以评估 mRNA 在发育中的大脑中的转化状态。

Tags

Polysome ProfilingTranslational RegulationBrain DevelopmentSucrose GradientEmbryonic Day 12Mouse CortexCycloheximideRNA Extraction

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