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Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures
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Ambiente
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JoVE Journal Ambiente
Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

Evaluating the Impact of Hydraulic Fracturing on Streams using Microbial Molecular Signatures

DOI:
10.3791/61904-v

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09:11 min

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April 04, 2021

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, , , , , ,
1Department of Biology,Juniata College, 2Wright Labs, LLC, 3Department of Biological Sciences,Michigan Technological University, 4Biosciences Division,Oak Ridge National Laboratory, 5Department of Civil and Environmental Engineering,University of Tennessee

Capítulos

  • 00:05Introduction
  • 00:42Sediment Sample Collection
  • 01:40Filter Collection
  • 03:02Nucleic Acid Extraction and Quantification
  • 05:17DNA 16S rRNA Library Creation
  • 05:55DNA 16S rRNA Library Purification
  • 06:46Results: Representative Hydraulic Fracturing Stream Microbial Molecular Signature Analyses
  • 08:23Conclusion

Summary

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Here, we present a protocol to investigate the impacts of hydraulic fracturing on nearby streams by analyzing their water and sediment microbial communities.

Tags

Keywords: Hydraulic FracturingStreamsMicrobialMolecular SignaturesSediment SamplesNucleic Acid Extraction16S DNA AnalysisMetatranscriptomics RNA AnalysisFilter CollectionPolyethersulfone FilterDNA/RNA Preservative

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