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El cultivo de una epidermis humana reconstruida tridimensional a gran escala
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
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El cultivo de una epidermis humana reconstruida tridimensional a gran escala

9,621 Views

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08:49 min

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May 28, 2021

DOI:

10.3791/61802-v

DOI:
10.3791/61802-v

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08:49 min
May 28, 2021

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37 Views
, , , , ,
1Adolphe Merkle Institute,University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences,University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute,North Carolina State University

Summary

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Este protocolo describe un método sencillo para cultivar epidermis humana reconstituida tridimensional de una manera reproducible y robusta. Además, caracteriza la relación estructura-función del modelo de barrera epidérmica. Las respuestas biológicas de la epidermis humana reconstituida sobre estímulos proinflammatory también se presentan.

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Cite this Article

Dijkhoff, I. M., Petracca, B., Prieux, R., Valacchi, G., Rothen-Rutishauser, B., Eeman, M. Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale. J. Vis. Exp. (171), e61802, doi:10.3791/61802 (2021).

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