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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

9,621 Views

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08:49 min

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May 28, 2021

DOI:

10.3791/61802-v

DOI:
10.3791/61802-v

•

08:49 min
May 28, 2021

•

37 Views
, , , , ,
1Adolphe Merkle Institute,University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences,University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute,North Carolina State University

Summary

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This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.

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Cite this Article

Dijkhoff, I. M., Petracca, B., Prieux, R., Valacchi, G., Rothen-Rutishauser, B., Eeman, M. Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale. J. Vis. Exp. (171), e61802, doi:10.3791/61802 (2021).

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