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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
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Bioengenharia
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JoVE Journal Bioengenharia
Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

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08:49 min

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May 28, 2021

DOI:

10.3791/61802-v

DOI:
10.3791/61802-v

•

08:49 min
May 28, 2021

•

16 Views
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1Adolphe Merkle Institute,University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences,University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute,North Carolina State University

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3D Epidermal ModelReconstructed Human Epidermis (RHE)Normal Human Epidermal Keratinocytes (NHEKs)Cell CultureIn VitroLarge-scale CultivationCell SeedingCell PassagingTrypsinizationCell SuspensionSubmerged CultureAir-liquid Interface Culture

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Dijkhoff, I. M., Petracca, B., Prieux, R., Valacchi, G., Rothen-Rutishauser, B., Eeman, M. Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale. J. Vis. Exp. (171), e61802, doi:10.3791/61802 (2021).

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