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高分解能融解解析を用いた CALR 遺伝子の遺伝的変異体検出

JoVE Journal
Genética

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JoVE Journal Genética

Genetic Variant Detection in the CALR gene using High Resolution Melting Analysis

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高分解能融解解析を用いた CALR 遺伝子の遺伝的変異体検出

4,395 Views

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08:46 min

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August 26, 2020

DOI:

10.3791/61642-v

DOI:

10.3791/61642-v

•

08:46 min

August 26, 2020

•

12 Views

Tadej Pajič^1,2, Tanja Belčič Mikič¹, Helena Podgornik^1,3, Jurka Klun¹, Sandra Šućurović¹, Samo Zver¹, Matjaz Sever¹

¹Department of Hematology,University Medical Centre Ljubljana, ²Clinical Institute for Genomic Medicine,University Medical Centre Ljubljana, ³Faculty of Pharmacy,University of Ljubljana

Summary

高分解能溶融解析(HRM)は、遺伝的変異体検出のための敏感で迅速なソリューションです。それは、ヘテロ二重鎖が融解曲線の形状を変化させる結果となるシーケンスの違いに依存する。HRMとアガロースゲル電気泳動を組み合わせて、インデルなどの異なる種類の遺伝的変異体を同定することができる。

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