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Biologia do Desenvolvimento
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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle

DOI:
10.3791/61625-v

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09:30 min

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July 08, 2020

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, , ,
1Reproductive and Developmental Biology Laboratory, Department of Health, Animal Science and Food Safety,University of Milan

Capítulos

  • 00:04Introduction
  • 00:50Media Preparation
  • 01:44Ovary Collection and Processing
  • 03:03Selection and Isolation of the Follicles and Retrieval of the COCs
  • 04:31Long in Vitro Culture of the Oocytes (long IVCO
  • 07:15Results: Meiotic Progression of Oocytes after long IVCO
  • 08:52Conclusion

Summary

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We describe the procedures for isolation of growing oocytes from ovarian follicles at early stages of development, as well as the setup of an in vitro culture system which can support the growth and differentiation up to the fully-grown stage.  

Tags

In Vitro CultureOocytesEarly Antral FolliclesCattleGenetic SalvageThreatened SpeciesLocal BreedsOvary SlicingFollicle SelectionCulture MediumCilostamideLong IVCO Medium96-well PlateIncubation Conditions

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