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JoVE Journal Immunology and Infection
Analysis of Human Natural Killer Cell Metabolism
 

Analysis of Human Natural Killer Cell Metabolism

Article DOI: 10.3791/61466-v • 09:03 min • June 22nd, 2020
June 22nd, 2020 •
1,2, 3, 3
1Cardiovascular Branch, National Heart, Lung and Blood Institute, National Institutes of Health, 2Departamento de Biología Molecular, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 3Lymphoid Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health

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Resumo
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In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.

Tags

Human Natural Killer Cell Metabolism Activation Oxygen Consumption PH Changes Extracellular Flux Analyzer High Throughput Screens Glycolysis Respiration Lymphocyte Separation Medium Blood Centrifuge Mononuclear Cells PBS NK Separation Buffer
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