Journal
/
Imunologia e Infecção
/
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

7,045 Views

•

10:36 min

•

January 09, 2018

DOI:

10.3791/56785-v

DOI:
10.3791/56785-v

•

10:36 min
January 09, 2018

•

2 Views
, , , , , , , , ,
1Department of Anatomical and Cellular Pathology,The Chinese University of Hong Kong, 2Li Ka Shing Institute of Health Sciences and Department of Medicine & Therapeutics,The Chinese University of Hong Kong

Summary

Automatically generated

Here, we present a protocol to mass-produce gene-silencing murine NK cells by using a feeder-free differentiation system for mechanistic study in vitro and in vivo.

Explore More Videos

Feeder-free SystemMass ProductionMurine Natural Killer CellsIn VitroInnate ImmunologyNatural Killer Cell DevelopmentCancer ImmunityBone MarrowPersonal ImmunotherapyOP9 CellsAlpha-MEM MediumPhosphate-buffered SalinePentobarbitoneFemur Bones

Read Article

Cite this Article

Tang, P. M., Tang, P. C., Chung, J. Y., Hung, J. S. C., Wang, Q., Lian, G., Sheng, J., Huang, X., To, K., Lan, H. A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro. J. Vis. Exp. (131), e56785, doi:10.3791/56785 (2018).

Download .ris file

Copy

Share Video

.

Copy