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小鼠胚胎干细胞中神经祖细胞和神经元的分化与表征
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
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小鼠胚胎干细胞中神经祖细胞和神经元的分化与表征

DOI:
10.3791/61446-v

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08:47 min

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May 15, 2020

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, , ,
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine,Penn State Hershey Cancer Institute

Capítulos

  • 00:04Introduction
  • 00:58EB, NPC, and Neuron Differentiation
  • 03:00Fixation and Embedding
  • 04:24Immunofluorescence
  • 05:45Results: E14 Differentiation into EBs, NPCs, and Neurons
  • 07:41Conclusion

Summary

Tadução automática

Tadução automática

我们描述了使用悬挂滴法将小鼠胚胎干细胞体外分化为神经元细胞的过程。此外，我们通过RT-qPCR，免疫荧光，RNA-seq和流式细胞术进行全面的表型分析。

Tags

Mouse Embryonic Stem CellsNeuron DifferentiationNeural ProgenitorsEctodermal DevelopmentHanging Drop CultureEmbryoid BodyNeural Progenitor CellsRetinoic AcidImmunocytochemistry

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